A-296 Validation of a Real Time PCR Kit for the Detection and Differentiation of MRSA, MSSA and MRCoNS in Respiratory Clinical Samples

Abstract Background Staphylococcus aureus infections are remarkable due to their high morbimortality. In addition, antibiotic resistance of these bacteria is a cause concern since it worsens the patient prognosis and associated higher morbidity, mortality cost treatment. The aim this study was assess clinical performance assay VIASURE Methicillin resistant Real Time Detection Kit in epidemiological respiratory samples. Methods Respiratory samples were collected from patients admitted intesive care or ward Hospital Royo Villanova (Zaragoza, Spain). A total 653 (both epidemiological) employed: 189 nasal swab, 103 pharyngeal, 161 bronchoalveolar aspirate (BAS), 154 lavage (BAL) 46 sputa previously characterised using culture methods. Results compared with other commercial validated molecular (RIDA®GENE MRSA (R-biopharm)) presenting same targets as under study. characterisation performed by based on combined results three S. SA 442/coA, mecA/mecC Sccmec-orfX junction may report following genotypes: methicillin-resistant (MRSA), methicillin-susceptible (MSSA)), coagulase negative (MRCoNS) coagulase-negative (MSSA MRCoNS). correctly reported MRSA/MSSA/MRCoNS genotyping sensitivity specificity values over 90% all sample matrixes (Table 1). Conclusion diagnostic demonstrated be suitable tool for diagnosis Staphylococci, methicillin causing mutation when reference